|
KMID : 0368420090520050426
|
|
Journal of Plant Biology
2009 Volume.52 No. 5 p.426 ~ p.426
|
|
|
Molecular Cloning and Expression of cDNA Encoding the Cysteine Proteinase Inhibitor from Upland Cotton
|
|
Jiang Ming-Feng
Li Sheng-Wei
Chen Min
Cai Ying-Fan
Xie Yong-Fang
Li Biao
Sun Quan
Jiang Huai-Zhong
Pan Zheng
Gao Yun-Ling
Yuan You-Lu
Shi Yu-Zheng
|
|
|
Abstract
|
|
|
|
A cDNA encoding a novel cysteine proteinase inhibitor (CPI) was isolated from a gland mutant Xiangmian-18 of upland cotton during the pigments gland forming stage. The cDNA comprises 378 bp and encodes 125 amino acid residues with molecular mass of 13.8 kDa. It contains the conserved motif of cysteine protease inhibitors and belongs to the cystatin superfamily (Gln-Val-Val-Ala-Gly). The deduced amino acid sequences of the domains are highly similar to the normal upland cotton (96.8%). SDS-PAGE and western hybridization analysis showed that the expressed recombinant protein was recombinant CPI. The inhibitory activity of recombinant CPI was 46 u/μg which was measured by inhibiting the protease activity of papain. RT-PCR results indicated that the expression level of developing gland stage was higher than that of undeveloped gland stage.
|
|
|
KEYWORD
|
|
|
Cysteine proteinase inhibitor, cDNA cloning, Expression, RT-PCR, Western blotting
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|